THE PHAGOCYTE WORKSHOP
ESCI MEETING IN UPPSALA APRIL 17-20, 2007
PRELIMINARY SCHEDULE
Tuesday April 17
17.00- Registration
19.00-20.00 Introductory lecture by Kari Stefansson
20.00- Welcome reception
Wednesday April 18
Session I: Development, maturation and ageing
8.30-9.05 Systems Biology of Neutrophil Development and Function
Professor Niels Borrgaard, Department of Hematology, University of Copenhagen, Rikshospitalet Copenhagen, Denmark
9.05-9.40 Membrane proteomes
Professor Gunnar von Heijne, Department of Biochemistry and Biophysics, Stockholm University,Sverige
9.40-9.55 EMR3: a new marker for fully differentiated neutrophils
A.J. Verhoeven1, M. Matmati2, W. Pouwels2, R. van Bruggen1, M. Janssen1 & J. Hamann2, 1Dept of Blood Cell Research, Sanquin Research and 2Dept. of Experimental Immunology, Academic Medical Center, Amsterdam, The Netherlands
9.55-10.10 The tetraspanin CD63 interacts with proelastase and is required for secretory lysosome formation in granulopoietic cells
1L. Källquist, 1M. Hansson, 1A-M. Persson, 2J. Calafat, 3H. Tapper & 1I. Olsson 1Department of Hematology, Lund, Sweden, 2Division of Cell Biology, The Netherlands Cancer Institute, Amsterdam, The Netherlands, and 3Department of Clinical Sciences, Lund, Sweden
10.10-10.30 Coffee break
10.30-11.30 ESCI PLENARY LECTURE
Session II: Molecular mechanisms of recognition
11.30-12.05 Phagocytosis and cell adhesion in Dictyostelium: integrins in amoebae?
Professor Pierre Cosson Departement de Physiologie Cellulare et Metabolisme, Centre Medical Universitaire, Geneva, Switzerland
12.5.12.20 The role of SBDS in neutrophil differentiation and migration
C. Orelio, J. Geissler, P. Verkuijlen, M. Jansen & T.W. Kuijpers. Dept. Blood Cell Research, Sanquin Research at CLB and Landsteiner Laboratory and Dept. Pediatrics, Emma Children's Hospital, AMC, Amsterdam, The Netherlands
12.20-12.35 Arsenic trioxide induces de novo synthesis of annexin-I in human neutrophils: link with heat shock but not apoptosis.
F. Binet. & D.Girard INRS-Institut Armand-Frappier, Pointe-Claire, Canada
12.35-12.50 FcRn: an IgG receptor on phagocytes with a novel role in phagocytosis
G. Vidarsson, A.M. Stemerding, N.M. Stapleton, F.E.M. Rebers, M. de Haas & J.G.J. van de Winkel. Dept. Experimental Immunohematology, Sanquin Research, and Landsteiner Laboratory, Academic Medical Center, University of Amsterdam, The Netherlands
12.50-13.05 The phagocyte-specific tyrosine kinase Hck is involved in lysosome propelling, podosome formation and transtissular migration
C. Vincent, A. Labrousse, R. Poincloux, C. Cougoule, V. Le Cabec & I. Maridonneau-Parini, CNRS UMR5089, Toulouse, France
13.00-14.00 Lunch
14.00-15.00 ESCI PLENARY LECTURE
15.00-15.35 Pathogen recognition by the apolipoprotein serum amyloid A
Professor John Raynes, Immunology Unit, Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London, UK
15.35-15.50 Serum amyloid A mediates human neutrophil production of reactive oxygen species through a G-protein coupled receptor independent of formyl peptide receptor like-1
L. Björkman* J. Karlsson*, H.M. Fu*, A. Karlsson*, M.J. Rabiet†, F. Boulay†, J Bylund* & C. Dahlgren*, *Department of Rheumatology and Inflammation Research, Göteborg University, Sweden and †DRDC/BBSI CEA-Grenoble, France
15.50-16.05 Soluble leukosialin (CD43s), released during the activation of neutrophils, inhibits their adhesion on endothelium under flow conditions
A. Mambole, D. Baruch§, P. Lesavre & L. Halbwachs-Mecarelli INSERM U 507, Hôpital Necker, §INSERM U 765, Paris, France
16.05-16.20 Neutrophil signalling via Fc? receptors
J.C Allen, R.J.Moots & S.W. Edwards School of Biological Sciences, University of Liverpool, Liverpool, United Kingdom
16.20-17.00 BUSINESS MEETING/DISCUSSION
17.00-18.00 Poster viewing and discussion
WE ARE PLANNING FOR THE PHAGOCYTE DINNER ON WEDNESDAY EVENING
Thursday April 19
Session III: Microbial pathogenesis
9.00-9.35 Innate immunity and resistance to kidney infections. A phagocyte perspective
Professor Catharina Svanborg Department of Clinical Immunology University of Lund, Sweden
9.35-9.50 Neutrophil expression of CD64 – a specific indicator of bacterial infection?
L. Hakansson1, G. Fjaertoft2, K. Pauksens3 & P. Venge1 Department of Medical Sciences, Sections of Clinical Chemistry1 and Infectious Diseases3 and Department of Women's and Children´s Health, Section of Paediatrics2, University Hospital, Uppsala, Sweden
9.50-10.05 Granulocyte transfusions: clinical effects and functional capacity of G-CSF-mobilized granulocytes
A. Drewniak1, J.J. Boelens2, H. Vrielink3, A.T.J. Tool1, M. van den Heuvel-Eibink4, L. Ball5, M.D. van de Wetering6, D. Roos1, A.J. Verhoeven1 & T.W. Kuijpers1,6 Sanquin Research and Landsteiner Laboratory, Amsterdam, 2Wilhelmina Children's Hospital, Utrecht, 3Sanquin Blood Bank, Northwest Region, Amsterdam; 4Sophia Children's Hospital, Rotterdam; 5Leiden University Medical Center, Leiden; 6Emma Children's Hospital AMC, Amsterdam, The Netherlands
10.05-10.30 Coffee break
10.30-11.30 ESCI PLENARY LECTURE
11.30-12.05 Inhibition of neutrophil function by Francisella tularensis
Professor Lee-Ann Allen, Inflammation Program Div of Infectious Diseases University of Iowa
12.05-12.20 P-Rex1 and Vav1 together regulate GPCR-dependent chemotaxis in neutrophils.
S. Donald & H. Welch The Babraham Institute, Cambridge, UK
12.20-12.35 Mitochondrial damage and caspase-independent death of neutrophils during primary SIV infection. Involvement in progression to AIDS.
C. Elbim, S. François, V. Monceaux, B. Hurtrel, MA. Gougerot-Pocidalo & J. Estaquier, INSERM, U773 - Service d'Immunologie et d'Hématologie, site Bichat, Paris, France; Institut Pasteur, Unité de Physiopathologie des Infections Lentivirales, Paris, France
12.35-12.50 Interactions of Haemophilus influenzae with neutrophils
D Bakstad, E. J. Naylor & S.W. Edwards, School of Biological Sciences, University of Liverpool, Liverpool, United Kingdom
13.00-14.00 Lunch
14.00-15.00 ESCI PLENARY LECTURE
Session IV: Phagocytosis and membrane dynamics
15.00-15.35 Neutrophil responses to pathogenic microbes
Professor Arturo Zychlinsky, Department of Cellular Microbiology, Max Planck Instritute for Infection Biology, Berlin, Germany
15.35-15.50 Hyperinflammatory responses of chronic granulomatous disease leukocytes – activation of NF-?B in the absence of reactive oxygen species
J. Bylund1,2, K. L. MacDonald2,3, K. L. Brown3, P. Mydel1,4, L. V. Collins1, R. E.W. Hancock3 & D. P. Speert,2,3 1Department of Rheumatology and Inflammation Research, Göteborg University, Sweden. 2Department of Pediatrics, 3Department of Microbiology and Immunology, University of British Columbia, Vancouver, Canada. 4Department of Microbiology, Jagiellonian University, Krakow, Poland
16.0.16.30 Coffee break
16.30-17.05 Lipid signalling and membrane remodeling during phagocytosis
Professor Sergio Grinstein, Division of Cell Biology, The Hospital for Sick Children, Toronto, Canada
17.15-17.30 Modulation of phagosome maturation by Streptococcus pyogenes bacteria
P. Nordenfelt, P. Lönnbro & H. Tapper, Clinical and Experimental Infection Medicine, Biomedical Centre, Lund University, Sweden
17.30-17.45 Localization of lipid rafts in phagocytosing human neutrophils
Martin E. Winberg1, Pontus Nordenfelt2, Per Lönnbro2, Birgitta Rasmusson1 & Hans Tapper2, 1Department of Molecular and Clinical Medicine, Division of Medical Biology, Linköping University, Linköping, Sweden; 2Section for Clinical and Experimental Infection Medicine, Department of Clinical Sciences, BMC, Lund University, Lund, Sweden
17.45-18.00 Heat shock proteins are involved in the initiation of an innate immune response against Mycobacterium tuberculosis infections
A. Persson & O. Stendahl, Division of Medical Microbiology, Department of Molecular and Clinical Medicine, Faculty of Health Sciences, Linköping University, Linköping, Sweden
ESCI CONFERENCE DINNER
Friday April 20
Session V: Killing Mechanisms
8.30-9.05 Endogenous antimicrobial peptides in fighting infections
Professor Birgitta Agerberth Department of Medical Biochemistry and Biophysics, KI, Stockholm, Sverige Birgitta.Agerberth@ki.se
9.05.9.20 Helical properties are important for the bactericidal effect of the antimicrobial peptide Hp(2-20) and can foresee antimicrobial actions of an Interleukin-8 derived peptide
Björstad, H. Fu, C. Dahlgren & J. Bylund, Department of Rheumatology and Inflammation Research, University of Göteborg, Sweden
9.20.9.35 Apoptosis-induced proteinase 3 membrane expression: association of proteinase 3 with phospholipid scramblase1
C. Kantari1*, M. Pederzoli-Ribeil1*, O. Amir-Moazami2, I. Cruz Moura2, M. Benhamou2 & V. Witko-Sarsat1 1INSERM U507, Necker Hospital, 2INSERM U699, Bichat Medical School, Paris, France *equally contributed to the work
9.35.9.50 Critical role of phospholipase C gamma 2 in neutrophils
Z. Jakus, T. Németh, Cs. Hably & A. Mócsai Department of Physiology, Semmelweis University School of Medicine, Budapest, Hungary
9.50-10.05 Open
10.00-10.30 Coffee break
10.30-11.30 ESCI PLENARY LECTURE
Session V Killing Mechanisms, continuous
11.30-12.00 The NADPH-oxidase and oxygen radical formation
12.0.12.15 Two new mutations in the CYBA and NCF2 genes leading to rare autosomal recessive chronic granulomatous diseases in Jordanian families
F. Bakri, M.S. El-Khateeb, C. Martel, F. Morel & M.J. Stasia Dept. of Medicine, Laboratory of Genetics, Jordan University Hospital, Amman, Jordan; GREPI EA-MENRT 2938, Université Joseph Fourier, Grenoble, France
12.15-12.30 Galectin-3 binds CD13, activates the NADPH-oxidase, and augments neutrophil inactivation of the chemotactic peptide fMLF
K. Granhagen, A. Lundström, A. Björstad, J. Bylund, A. Karlsson & C. Dahlgren Department of Rheumatology and Inflammation Research, Göteborg University, Sweden
12.30-13.00 CONCUDING REMARKS BY THE OLD AND NEW ORGANIZERS
13.00-14.00 Lunch
II Workshops
Theme: Inflammation
1. Phagocyte biology: from gene to function
C. Dahlgren (Göteborg,
Sweden), A. Karlsson (Göteborg, Sweden)
2. Inflammation and autoimmunity
L. Rönnblom (Uppsala, Sweden)
3. Transplantation and innate immunity
G. Tufveson (Uppsala, Sweden), O.
Korsgren (Uppsala, Sweden)
4. Asthma and COPD: genetics, remodelling and therapeutic
consequences
C. Janson (Uppsala, Sweden), P. Venge (Uppsala, Sweden)
Theme: Cardiovascular disease
5. Arteriosclerosis: basic mechanisms and clinical consequences
B.
Lindahl (Uppsala, Sweden), G. Hansson (Stockholm, Sweden), B. Fellström (Uppsala, Sweden)
6. Clinical pharmacogenetics
M.L. Dahl (Uppsala, Sweden), H. Melhus (Uppsala, Sweden)
Theme: Cancer
7. Cancer - basic mechanisms
P.Venge (Uppsala, Sweden)
8. Urinary bladder cancer
P.U. Malmström (Uppsala, Sweden)
9. Colorectal cancer
B. Glimelius (Uppsala, Sweden), L. Pahlgren
(Uppsala, Sweden)
10. Lymphoma: genetics and inflammation
G. Enblad (Uppsala, Sweden)
11. Current status of individualized pharmacotherapy of cancer
R. Larsson
(Uppsala, Sweden), P. Nygren (Uppsala, Sweden)
Theme: Metabolism
12. Diabetes type II and obesitas
A.F. Karlsson (Uppsala, Sweden), C.
Berne (Uppsala, Sweden)
13. Nutrition, alcoholic liver disease and NASH
P. Stärkel (Brussels, Belgium)
Theme: Amyloids, Alzheimer's disease and Traumatic brain injury
14. Neurodegenerative disorders - Alzheimer's disease
L. Lannfelt (Uppsala, Sweden)
15. Amyloids and prions in disease
P. Westermark (Uppsala, Sweden) -
16. Traumatic brain injury - from molecule to man
L. Hillered (Uppsala, Sweden), P. Enblad (Uppsala, Sweden
Theme: Future tools in molecular medicine
17. Future tools in molecular medicine
U. Landegren (Uppsala, Sweden), A.C. Syvanen (Uppsala, Sweden)
Theme: Global epidemics
18. Antibiotic resistance
D. Andersson (Uppsala, Sweden), O. Cars (Stockholm, Sweden